<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Lei Li</style></author><author><style face="normal" font="default" size="100%">Chung-Yen Oliver Chen</style></author><author><style face="normal" font="default" size="100%">{Hye-Kyung} Chun</style></author><author><style face="normal" font="default" size="100%">{Soo-Muk} Cho</style></author><author><style face="normal" font="default" size="100%">{Ki-Moon} Park</style></author><author><style face="normal" font="default" size="100%">Yang Cha {Lee-Kim}</style></author><author><style face="normal" font="default" size="100%">Jeffrey B Blumberg</style></author><author><style face="normal" font="default" size="100%">Robert M Russell</style></author><author><style face="normal" font="default" size="100%">Kyung-Jin Yeum</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A fluorometric assay to determine antioxidant activity of both hydrophilic and lipophilic components in plant foods</style></title><secondary-title><style face="normal" font="default" size="100%">The Journal of Nutritional Biochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Amidines</style></keyword><keyword><style  face="normal" font="default" size="100%">Angelica</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidants</style></keyword><keyword><style  face="normal" font="default" size="100%">Azo Compounds</style></keyword><keyword><style  face="normal" font="default" size="100%">Boron Compounds</style></keyword><keyword><style  face="normal" font="default" size="100%">Chemical Fractionation</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluoresceins</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitriles</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants</style></keyword><keyword><style  face="normal" font="default" size="100%">Solubility</style></keyword><keyword><style  face="normal" font="default" size="100%">Vegetables</style></keyword><keyword><style  face="normal" font="default" size="100%">{Oxidation-Reduction</style></keyword><keyword><style  face="normal" font="default" size="100%">} Perilla</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">03/2009</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/18602808</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">3</style></number><volume><style face="normal" font="default" size="100%">20</style></volume><pages><style face="normal" font="default" size="100%">219–226</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">This study aimed to develop a fluorometric method to determine total antioxidant activity of plant foods. The antioxidant activities in plant foods were determined after extracting (1) hydrophilic components with acidified methanol (methanol:glacial acetate acid:water=50:3.7:46.3), (2) lipophilic components with methanol followed by tetrahydrofuran {(THF),} or (3) both hydrophilic and lipophilic components using sequential extraction of acidified methanol and {THF} together. Both the hydrophilic assay [using the hydrophilic radical initiator 2,2'-azobis-(2-amidinopropane)dihydrochloride (10 {mmol/L)} and hydrophilic probe 2,7-dichlorodihydrofluorescein {(DCFH)]} and the lipophilic assay [using the lipophilic radical initiator [2,2'-azobis (4-methoxiy-2,4-dimethylvaleronitrile), 2 {mmol/L],} and the lipophilic probe 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid {(C11-BODIPY} 581/591) {(BODIPY:} 2 {micromol/L)]} were used to measure antioxidant activity. The inhibition of {BODIPY} oxidation was significantly increased {(P{\textless}.01)} when both the hydrophilic and lipophilic components were extracted using acidified methanol and organic solvent as compared to those extracted by organic solvent alone. In addition, the rate of {DCFH} oxidation was significantly delayed {(P{\textless}.05)} when both components coexisted compared to {DCFH} oxidation of the hydrophilic component alone. The combination of lipophilic and hydrophilic components in these plant foods showed significantly greater antioxidant activity than that of either hydrophilic or lipophilic component alone. Thus, both hydrophilic and lipophilic components in plant foods and their interactions should be considered when determining their antioxidant activity.</style></abstract><notes><style face="normal" font="default" size="100%">{PMID:} 18602808</style></notes></record></records></xml>