<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Merav E Shaul</style></author><author><style face="normal" font="default" size="100%">Grace Bennett</style></author><author><style face="normal" font="default" size="100%">Katherine J Strissel</style></author><author><style face="normal" font="default" size="100%">Andrew S Greenberg</style></author><author><style face="normal" font="default" size="100%">Martin S Obin</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Dynamic, M2-like remodeling phenotypes of CD11c+ adipose tissue macrophages during high-fat diet–induced obesity in mice</style></title><secondary-title><style face="normal" font="default" size="100%">Diabetes</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Adipose Tissue</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Antigens</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Surface</style></keyword><keyword><style  face="normal" font="default" size="100%">Dietary Fats</style></keyword><keyword><style  face="normal" font="default" size="100%">Differentiation</style></keyword><keyword><style  face="normal" font="default" size="100%">Flow Cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Immunohistochemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Inbred {C57BL</style></keyword><keyword><style  face="normal" font="default" size="100%">Interleukin 1 Receptor Antagonist Protein</style></keyword><keyword><style  face="normal" font="default" size="100%">Interleukin-12 Subunit p40</style></keyword><keyword><style  face="normal" font="default" size="100%">Interleukin-13</style></keyword><keyword><style  face="normal" font="default" size="100%">Macrophages</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Myelomonocytic</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Receptors</style></keyword><keyword><style  face="normal" font="default" size="100%">{CD</style></keyword><keyword><style  face="normal" font="default" size="100%">{CD11c</style></keyword><keyword><style  face="normal" font="default" size="100%">{CD86</style></keyword><keyword><style  face="normal" font="default" size="100%">} Antigens</style></keyword><keyword><style  face="normal" font="default" size="100%">} Obesity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">05/2010</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/20185806</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">5</style></number><volume><style face="normal" font="default" size="100%">59</style></volume><pages><style face="normal" font="default" size="100%">1171–1181</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">OBJECTIVE To identify, localize, and determine {M1/M2} polarization of epidydimal adipose tissue (eAT macrophages (Phis) during high-fat diet (HFD)-induced obesity
RESEARCH DESIGN AND METHODS Male C57BL/6 mice were fed an HFD (60% fat kcal) or low-fat diet {(LFD)} (10% fat kcal) for 8 or 12 weeks. {eATMPhis} {(F4/80(+)} cells) were characterized by in vivo fluorescent labeling, immunohistochemistry, fluorescence-activated cell sorting, and quantitative PCR.
RESULTS Recruited interstitial macrophage galactose-type C-type lectin {(MGL)1(+)/CD11c(-)} and crown-like structure-associated {MGL1(-)/CD11c(+)} and {MGL1(med)/CD11c(+)} eATMPhis were identified after 8 weeks of {HFD.} {MGL1(med)/CD11c(+)} cells comprised approximately 65% of {CD11c(+)} {ATMPhis. CD11c(+) eATMPhis expressed a mixed {M1/M2} profile, with some M1 transcripts upregulated {(IL-12p40} and {IL-1beta),} others downregulated {(iNOS,} caspase-1, {MCP-1,} and {CD86),} and multiple M2 and matrix remodeling transcripts upregulated (arginase-1, {IL-1Ra,} {MMP-12,} {ADAM8,} {VEGF,} and Clec-7a). At {HFD} week 12, each {eATMPhi} subtype displayed an enhanced M2 phenotype as compared with {HFD} week 8. {CD11c(+)} subtypes downregulated {IL-1beta} and genes mediating antigen presentation {(I-a,} {CD80)} and upregulated the M2 hallmark Ym-1 and genes promoting oxidative metabolism {(PGC-1alpha)} and adipogenesis {(MMP-2).} {MGL1(med)/CD11c(+)} {eATMPhis} upregulated additional M2 genes {(IL-13,} {SPHK1,} {CD163,} {LYVE-1,} and {PPAR-alpha).} {MGL1(med)/CD11c(+)} {ATMPhis} expressing elevated {PGC-1alpha,} {PPAR-alpha,} and Ym-1 transcripts were selectively enriched in {eAT} of obese mice fed pioglitazone for 6 days, confirming the M2 features of the {MGL1(med)/CD11c(+)} {eATMPhi} transcriptional profile and implicating {PPAR} activation in its elicitation.
CONCLUSIONS These results 1) redefine the phenotypic potential of {CD11c(+)} {eATMPhis} and 2) suggest previously unappreciated phenotypic and functional commonality between murine and human {ATMPhis} in the development of obesity and its complications.</style></abstract><notes><style face="normal" font="default" size="100%">{PMID:} 20185806</style></notes></record></records></xml>